Technical Data Sheet Biotin Hamster Anti-Mouse CD11c
Material Number: Alternate Name: Size: Concentration: Clone: Immunogen: Isotype: Reactivity: Storage Buffer:
553800 Integrin αx chain
HL3 C57BL/6 Mouse Intestinal Intraepithelial Lymphocytes Armenian Hamster IgG1, λ2 QC Testing: Mouse
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
Description The HL3 antibody reacts with the integrin αx chain of gp150, 95 (CD11c/CD18) which is expressed on dendritic cells and CD4- CD8+ intestinal intraepithelial lymphocytes (IEL) and is upregulated on IEL and lymph-node T cells following in vivo activation. CD11c is also found on human NK cells. Although its expression on mouse NK cells is not published, we have detected CD11c on mouse splenic NK cells. Cells of the monocyte/macrophage lineage have been reported to express low levels of CD11c. CD11c plays a role in binding of iC3b.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Expression of CD11c on spleen NK cells. C57BL/6 splenocytes were stained simultaneously with PE-conjugated anti-mouse NK-1.1 mAb PK136 (Cat. No. 557391/553165) and isotype control (left panel) or biotin-conjugated mAb HL3 (right panel), followed by Avidin-FITC (Cat. No. 554057). Flow cytometry was performed on a BD FACScan™ flow cytometry system.
Preparation and Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application Notes Application Flow cytometry
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Size 0.5 mg