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PCR:  The Steps of the Process

3 phases for each cycle (these vary slightly from one protocol to another)

Denaturing:  (94-95°C) DNA strands separate into single strands

Annealing:  (58°C) Primers anneal (attach) to the separated DNA strands

Extending:  (72°C) New complementary strands are made as the Taq enzyme helps to form bonds with the DNTP’s

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