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Steps of DNA Isolation:

10 mL of saline, swish, spit, use 1mL of this.

Centrifuged

Poured off saline & then resuspended

Removed 30 uL and added to 100 uL of Chelex to absorb cell debris and ions.

Boiled for 10 minutes.

Withdrew 30 uL & stored in new tube.

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