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Walk On The Y Concept:

A sequencing run may, or may not be successful

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    Sequence may not exist in this Y chromosome

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    Primer binding site has a mutation

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    3D folding of DNA may make region inaccessible

To keep costs low, the sequencing run will NOT be repeated

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    Report just says “failed segment”

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    A failed segment will be rewarded as a new segment in a future contig

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    Customer has option to re-order failed segment as individual segment

We want to step forward to cover large regions in a cost effective way Later we come back and look at the details.

A PCR microplate has 8 x 12 = 96 wells Segments are 500-700 bp long Overlap needed at the ends of segments 500 bp x 96 = 48.000 bp 2 plates needed for forward & reverse sequencing

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