X hits on this document





21 / 24


In sum, there is a unique risk posed by SCNT clones that is not posed by other ARTs (artificial reproductive technologies): the potential for aberrant nuclear- mitochondrial interactions. Embryos created via SCNT embryos differ from those created by other ARTs in two ways: they may be heteroplasmic (e.g. contain mtDNA from two sources) and nuclear-encoded mitochondrial DNA transcription and translation factors persist in SCNT, but not IVF, embryos. Given that there is a long list of diseases that are influenced by aberrant mitochondia, any aberrant nuclear-mitochondrial interaction, due to heteroplasmy and/or persistence of mitochondrial transcription and translation factors in the SCNT clones, should raise red flags that deserve more study. The area of mitochondrial diseases is an emerging field and there is a lot of information that is still unknown. Until the FDA understands more about nuclear-mitochondrial cross talk and the impact of heteroplasmy, they should refrain from approving SCNT clones.


Anonymous. 2005. The problem with clones. New Scientist 2472: 20. At: http://www.newscientist.com/channel/sex/mg18424722.700.

Anonymous. 2007. Dolly: a hard act to follow. Nature 445: 802.

Aoki S, Takahashi R, Nisisouzu T, Kitamura S, Dochi O, Kishi M, Morita S, Komiya M, Tarawaki Y and H Hoyama. 2003. A comparative investigation of the characteristics of Holstein cows cloned from colostrum-derived mammary grand epithelial cells in an automatic milking system [ABSTRACT ONLY]. Theriogenology 59: 234

Archer, G.S., Dindot, D., Friend, T.H., Walker, S., Zaunbrecher, G., Lawhorn, B. and J.A. Piedrahita. 2003. Hierarchical phenotypic and epigenetic variation in cloned swine. Biology of Reproduction, 69: 430-436.

Batchelder, CA. 2005. Cloning in Cattle: Effect Of The Nuclear-Donor Cell On Cloning Efficiency, Perinatal Physiology, and Long-Term Health of Cloned Calves.

Campbell, K.H., McWhir, J., Ritchie, W.A. and I. Wilmut. 1996. Sheep cloned by nuclear transfer from a cultured cell line. Nature, 380: 64-66.

Carroll, J, Carter, DB, Scott, K and P Randall. 2005. Evaluation of the acute phase response in cloned pigs following a lipopolysaccharide challenge. Domest Animal Endocrinology 29(3): 564-572.

Do JT, Lee JW, Lee BY, Kim SB, Ryoo ZY, Leet HT and KS Chung. 2002. Fate of donor mitochondrial DNA in cloned bovine embryos produced by microinjection of cumulus cells. Biology of Reproduction 67: 555-560.

Food and Drug Administration (FDA). 2006. Draft Animal Cloning Risk Assessment1

Document info
Document views52
Page views52
Page last viewedTue Oct 25 12:48:32 UTC 2016