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Sequence Analysis of Single Polymorphism in the Factor V Gene - page 15 / 15

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Biol  420                                                                                                                                          Fall 2007

Discussion Questions

1.

Describe your results.  What do they tell you about the status of your factor V gene?

2.

Discuss the usefulness of sequencing as a tool for genetic analysis, particularly, in disease research.  Are there specific types of genetic diseases that this type of sequencing is not applicable?  Explain.

3.

With modern DNA sequencing techniques it is now feasible to sequence all the DNA in a human cell.  Social, moral and ethical considerations can, however, be raised.  Should such a major sequencing endeavor be encouraged?

4.

The following gel pattern was obtained in an attempt to sequence a DNA molecule using the Sanger method.  What is the sequence of the DNA molecule in questions?

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