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Detection Of Viral Pathogens by Culture

Viruses are obligate intracellular parasites

Some viruses can be propagated or cultured in susceptible hosts

whole animals

mammalian cells grown in culture

Quantify viruses in animals using quantal methods (e.g., Most Probable Number or MPN); in eggs using “pock” assays on egg membranes (smallpox and vaccinia initially enumerated this way)

Virus assays in cell cultures by quantal (e.g., MPN) or enumerative methods (plaque or local lesion assays)

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