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Challenges in Assaying Viral Infectivity

Host susceptibility and variability in host susceptibility

Type of host cells and cell culture assay methods:

Plaque (enumerative) assays

Quantal (liquid culture) assays

Quantal assay endpoints:

»

Cytopathogenic effects:  visible changes in cells

»

Immunodetection or nucleic acid detection

Animal bioassays:  used if no cell culture assay available

Virus titer often increases with serial passages in hosts

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