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Some viruses (some enteroviruses, adenoviruses, rotaviruses, astroviruses and hepatitis A virus) grow poorly or slowly in cell cultures and produce little or no CPE.

Greater detection with additional analytical techniques:

Viral antigens

Immunofluorescence assays, enzyme immunoassays, radioimmunoassays, etc.

Viral nucleic acid assays: hybridization and/or amplification

Combined cell culture + RT-PCR demonstrates presence of greater numbers of infectious viruses than CPE alone

Post-disinfection, more viruses are detected than by CPE

Challenges in Assaying Viral Infectivity

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