Detection of Protozoan Parasites by Culture
Environmental forms of some protozoan parasites, such as spores and oocysts, are culturable in susceptible host cells
Culture Cryptosporidium parvum oocysts and some microsporidia spores in mammalian cell cultures; observe living stages
Culture free-living amoebas (Naegleria spp. and Acanthamoeba spp.) on lawns of host bacteria, such as E. coli, on nonnutrient agar; they form local lesions.
For other waterborne parasites, such as Giardia lamblia and Cyclospora cayatenensis, culture from the environmental stage (the cyst or oocyst) is still not possible