Detection of Protozoan Parasites by Culture
Spores of some microsporidia (Encephalitozoon intestinalis) and the oocysts of Cryptosporidium parvum can be cultured in mammalian host cells where spores germinate or oocysts excyst and active stages of the organisms can proliferate.
Living stages detected (after immunofluorescent or other staining) and quantified: score positive and negative microscope fields or cell areas (slide wells), or count numbers of foci of living stages or discrete living stages.
Express concentrations MPNs or other units, such as numbers of live stages.
Detection of living stages also possible by FISH, PCR or immunoblotting
Facilitates molecular characterization