P16: Evaluation of antagonistic effects of some intestinal bacterial normal flora on Salmonella paratyphi A,B,C under invitro condition, S. Assar
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Evaluation of antagonistic effects of some intestinal bacterial normal flora on Salmonella paratyphi A,B,C under invitro condition
In bacterial growth studies, we observe a series of complex factors influencing the antagonistic, symbiosis and perhaps the whole range of interrelations between microbial species.
Because of the increasing resistance of pathogenic bacteria, treatment of infectious diseases become increasingly difficult and if we design a study to show inhibitory effect of some bacteria against the others, we may achieve a way to control some pathogenic bacteria and consequently, infectious diseases.
We may use some techniques (flip streak assay with time lap, well diffusion assay, spot in the lawn and.......) to recognize, how an antagonistic effect will occur .
We will use some culture media & about 1000 isolated colonies from non-diarrheal healthy volunteer individuals and examine each of them against Salmonella paratyphi A,B,C .
Then we compare all methods, which are used for investigation of mentioned antagonistic effect. In the same time, we may also interact assumed antagonist bacteria in liquid condition. Techniques that will be used for this reason are:
a) Mixture of antagonist's supernatant&Salmonella paratyphi's broth culture.
b) Mixture of antagonist's supernatant&freshly inoculated Salmonella paratyphi's broth culture.
c) Mixture of antagonist's broth&Salmonella paratyphi's broth.
d) Synchronous inoculation of antagonist bacteria&Salmonella paratyphi into a fresh broth
We practice all above techniques and incubate all media at 37C for 24 hours, then inoculate a batch of broth assays to appropriate media. Since these bacteria(assumed antagonist bacteria) are intestinal normal flora, they may inhibit substitution and colonization of Salmonella paratyphi, that is an important pathogenic bacteria of man.
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