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618 : 2002 Vol 43(12) Singapore Med J

Normal

196 10.40

208 9.80

97.50 8.04a

16.03 1.04a

12.85 0.72a

0.22 0.01a

Nil

Diabetic control

201 15.70

151 13.66•••

232.00 15.40b

4.35 0.95b

5.60 0.45b

0.81 0.07b

+++

Diabetic + Cassia auriculata (0.15g/kg)

193 17.70

198 15.33***

216.66 20.80b

4.90 0.41b

6.91 0.61c

0.68 0.03c

++

Diabetic + Cassia auriculata (0.30g/kg)

198 18.30

208 10.32***

158.60 14.20c

7.05 0.64c

9.54 0.93d

0.48 0.04d

+

Diabetic + Cassia auriculata (0.45g/kg)

202 19.68

214 12.72***

113.3 10.30ad

14.16 0.67d

11.5 0.91e

0.37 0.04e

NIL

Diabetic + Glibenclamide (600 g/kg)

195 11.80

206 13.43***

124.6 10.32d

12.70 0.65e

10.36 1.01d

0.47 0.04d

TRACE

Body weight (g)

Fasting Blood

Plasma

Haemoglobin

Glycosylated

Urine

Initial

Final

Glucose

insulin

(g/dl)

haemoglobin

sugarA

(mg/dl)

(U/ml)

(mg/gHb)

Table I. Blood glucose, plasma insulin, total haemoglobin, glycosylated haemoglobin, changes in body weight and urine sugar of normal and experimental animals.

Groups

Values are given as mean S.D for six rats in each group. Values not sharing a common superscript letter differ significantly at p<0.05 (DMRT). Duncan procedure, Range for the level 2.89, 3.03, 3.13, 3.20, 3.25.

Diabetic control was compared with normal, ••• p<0.001. Experimental groups were compared with diabetic control, ***

p<0.001.

A - Indicates 0.25% sugar and (+ + +) indicates more than 1% sugar.

Groups

Cholesterol

Free fatty acids

Triglycerides

Phospholipids

(mg/100 ml)

(mg/100 ml)

(mg/100 ml)

(mg/100 ml)

Normal

74.00 1.49a

69.43 4.06a

44.53 3.36a

80.25 1.57a

Diabetic control

98.66 4.03b

83.86 6.67b

62.83 1.50b

98.75 4.28b

Diabetic + Cassia auriculata (0.45 g/kg)

83.46 2.18c

75.06 1.55c

53.93 2.70c

85.50 2.86c

Diabetic + Glibenclamide (600 g/kg)

90.26 1.37d

78.51 0.87d

58.46 1.70d

90.00 2.12d

Table II. Changes in levels of cholesterol, free fatty acids, triglycerides and phospholipids in serum of normal and experimental animals.

Values are given as mean S.D for six rats in each group. Values not sharing a common superscript letter differ significantly at p<0.05 (DMRT). Duncan procedure, Range for the level 2.95, 3.09, 3.20.

plant was identified and authenticated at the Herbarium of Botany Directorate in Annamalai University. A voucher specimen (No.231) was deposited in the Botany Department of Annamalai University.

surviving rats, six normal rats) were used. The rats

were divided into six groups of six rats each. Group 1: Normal untreated rats. Group 2: Diabetic control rats given 1 ml of aqueous

Preparation of plant extract Five hundred g of Cassia auriculata flowers were extracted with 1,500 ml of water by the method of continuous hot extraction at 60ºC for six hours and evaporated. The residual extract was dissolved in water and used in the study(9).

Induction of experimental diabetes A freshly prepared solution of streptozotocin (45 mg/kg i.p) in 0.1 M citrate buffer, pH 4.5 was injected intraperitoneally in a volume of 1 ml/kg. After 48 hours of streptozotocin administration, rats with moderate diabetes having glycosuria and hyperglycaemia (i.e. with a blood glucose of 200- 300 mg/dl) were taken for the experiment(10).

Experimental procedure In the experiment, a total of 36 rats (30 diabetic

solution daily using an intragastric tube for 30 days. Group 3: Diabetic rats given CFEt (0.15 g/kg body

weight) in 1ml of aqueous solution daily using an intragastric tube for 30 days. Group 4: Diabetic rats given CFEt (0.30 g/kg body

weight) in 1 ml of aqueous solution daily using an intragastric tube for 30 days. Group 5: Diabetic rats given CFEt (0.45 g/kg body

weight) in 1 ml of aqueous solution daily using an intragastric tube for 30 days. Group 6: Diabetic rats given glibenclamide (600 g/

kg body weight)(11) in 1 ml of aqueous solution daily using an intragastric tube for 30 days. At the end of 30 days, the animals were deprived of food overnight and sacrificed by decapitation. Blood was collected in two different tubes (i.e.,) one with anticoagulant- potassium oxalate and sodium fluoride for plasma and another without

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