Singapore Med J 2002 Vol 43(12) : 619
Cholesterol (mg/100 g wet tissue)
Free fatty acids (mg/100 g wet tissue)
Triglycerides (mg/100 g wet tissue)
Phospholipids (g/100 g wet tissue)
Normal Diabetic control
329.04 2.88a 512.70 5.88b
607.70 30.68a 915.22 50.27b 774.09 46.86c 806.67 25.30c
347.88 13.04a 621.35 8.40b 442.98 13.05c 530.19 11.70d
1.66 0.11a 2.54 0.08b 2.02 0.05c 2.29 0.10d
Diabetic + Cassia auriculata (0.45 g/kg) Diabetic + Glibenclamide (600 g/kg)
420.14 4.40c 441.98 5.36d
Table III. Changes in levels of cholesterol, free fatty acids, triglycerides and phospholipids in liver of normal and experimental animals.
Values are given as mean S.D for six rats in each group. Values not sharing a common superscript letter differ significantly at p<0.05 (DMRT). Duncan procedure, Range for the level 2.95, 3.09, 3.20.
Hexokinase (unitsA/g protein)
Glucose- 6-phosphatase (unitsB/mg protein)
146.66 6.09a 107.48 5.74b 128.70 9.44c 123.20 5.40c
0.168 0.013a 0.242 0.023b 0.186 0.011ac 0.200 0.008c
Table IV. Changes in activities of hexokinase and glucose-6-phosphatase in liver of normal and experimental animals.
Normal Diabetic control Diabetic + Cassia auriculata (0.45 g/kg) Diabetic + Glibenclamide (600 g/kg)
Values are given as mean S.D for six rats in each group. Values not sharing a common superscript letter differ significantly at p<0.05 (DMRT). Duncan procedure, Range for the level 2.95, 3.09, 3.20. A - moles of glucose phosphosylated/min. B - moles of Pi liberated/min.
anticoagulant for serum separation. Plasma and serum were separated by centrifugation.
by ANOVA followed by Duncan’s Multiple Range Test (DMRT)(22).
Liver was immediately dissected out, washed in ice cold saline, patted dry and weighed.
Analytical Procedure Fasting blood glucose was estimated by O-toluidine method (Sasaki et al)(12). Plasma insulin level was assayed by Enzyme Linked Immunosorbent Assay (ELISA) kit, using human insulin as standard. Haemoglobin was estimated by the method of Drabkin and Austin(13) and glycosylated haemoglobin by the method of Sudhakar Nayak and Pattabiraman(14). Lipids were extracted from serum and tissues by the method of Folch et al(15). Total cholesterol and triglycerides were estimated by the method of Zlatkis et al(16) and Foster and Dunn(17) respectively. Free fatty acids and phospholipids were analysed by the method of Falholt et al(18) and Zilversmit et al(19).
RESULTS Blood glucose and Plasma insulin Table I shows the levels of blood glucose, plasma insulin, total haemoglobin, glycosylated haemoglobin, changes in body weight and urine sugar of normal and experimental rats. There was a significant elevation in blood glucose and glycosylated haemoglobin levels, while the plasma insulin and total haemoglobin levels decreased significantly in streptozotocin diabetic rats when compared with normal rats. Administration of CFEt and glibenclamide tends to bring the parameters significantly towards the normal. The effect of CFEt at a dose of 0.45 g/kg body weight was more highly significant than 0.15 and 0.30 g/kg body weight and therefore the dose was used for further biochemical studies.
Hexokinase and glucose-6-phosphatase were assayed by the method of Brandstrup et al(20) and Koida and Oda(21).
Statistical analysis All values were expressed as the mean obtained from a number of experiment (n). Data from all the tables of normal animals, diabetic control animals, reference drug treated and CFEt treated animals were compared
In diabetic rats, the urine sugar was (+++) but in the case of CFEt treated rats at a dose of 0.15 and 0.30 g/kg body weight showed decreased urine sugar (++) and (+) respectively. CFEt at a dose of 0.45 g/kg body weight, showed urine sugar as seen in normal
rats. These effects were compared with glibenclamide.
Serum and tissue lipids The effect of CFEt on serum and tissue lipids of