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Micro Fluorescence Actuated Cell Sorter - page 11 / 30

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1/23/2015

ME 595

11

OPTICAL DETECTION SYSTEM

Laser emitting device integrated with collimating lenses to control the polarization of the beam, focused on to the flow through an inverted microscope for illumination.

A slit or window on the microscope through which the emitted and scattered light is collected and then focused on to a photo multiplier tube and ccd camera using beam splitters.

The optical systems also contain means to enhance light intensities to increase the resolution and sensitivity of the optical system.

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