FLOW MANIPULATING SCHEMES
Reverse Sorting Scheme
-Involves maintaining a flow between the input and waste channels until preset threshold fluorescence is achieved .
-Once a target cell is detected the flow is stopped and reversed, as the cell must have already passed into the waste channel.
- Speed of the reversed flow is maintained at slow switch able speed and once target cell is detected again, flow direction is changed to direct the target cell into collection channel
Anne Y. Fu et al, Nature Biotechnology Vol. 17(1999)