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Russian regional scientists project proposals for participation in FP7 - page 4 / 62

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4

THEME 1: Health

1.2. Translating research for human health

4. Prof. Tatyana N. Popova

Organization Name: Voronezh State University

Position in Organization: The head of the Department of Analytical and Medical Biochemistry

and Microbiology of Biology and Soil Science Faculty

E-mail address: tpopova@bio.vsu.ru

Telephone: +7 –4732 – 20-82-78

Address: Voronezh State University 1, Universitetskaya pl.,394006, Voronezh, Russian Federation

Proposal/Expertise: Research of the oxidative status and oxidative metabolism enzymes activity in mammals tissues at pathologies, connected with oxidative stress, and protectors action

Description of proposal or expertise:

Abstract: The activity is directed on the decision of a fundamental problem of biochemistry connected to research of functioning of antioxidant system (АОS) and oxidative metabolism enzymes, capable to limit free-radical oxidation (FRО), at pathologies, connected with oxidative stress (experimental models of liver toxic defeat, myocardium and brain ischemia, hyperthyroidism at rats), and also at action of substances-protectors (thioctic acid, citrate, melatonin and several synthetic substances, received at Voronezh State University Chemical Faculty). Estimation of free-radical processes intensity in analysed tissues is realised by biochemiluminescence method, giving the integral characteristic of a FRO level, definition of the contents of lipid peroxidation primary products – diene conjugates, and DNA fragmentation as index of apoptosis development degree. Activity of AOS enzymes (superoxide dismutase, catalase, glutathione reductase and glutathione peroxidase) and level of some antioxidants (-tocopherol, citrate, glutathione) in liver, myocardium, brain and blood serum at pathologies and protectors action are investigated. The research of aconitase (АH), succinate dehydrogenase (SDH), citrate synthase (CS), NAD- and NADP-isocitrate dehydrogenases (NAD-IDH and NADP-IDH), NAD- and NADP-malate dehydrogenases (NAD-MDH and NADP-MDH), glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6PGDH) functioning with application of purified enzyme preparations is also spent. The research of these enzymes expression and repression regulation is planed.

The investigation of biochemical bases of reactive oxygen species formation regulation with the help of substances-protectors promotes clarification of molecular mechanisms of FRO intensity increase, which is a universal non-specific link in various diseases pathogenesis, prevention.

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