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Chapter 8*: SANITARY INSPECTION AND MICROBIOLOGICAL WATER QUALITY - page 15 / 52

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Figure 8.2 Inoculation scheme for the multiple test tube method

After the incubation period, all the inoculated presumptive positive tubes must be inoculated into a more selective medium to confirm the presence of the target bacteria (confirmatory phase). The confirmation test is reliable evidence, but not proof, that the target bacteria have been detected. Therefore, subsamples of the confirmed positive reactions should be inoculated onto a selective agar medium and several verification tests (Gram stain, and biochemical, serological or enzymatic tests) should be carried out (Tables 8.3 and 8.4). This completed test is generally conducted on 10 per cent of the positive tubes as a quality control measure. For practical purposes, the number of

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