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Chapter 14 APPLICATION OF ULTRAVIOLET/VISIBLE MOLECULAR ABSORPTION SPECTROMETRY - page 24 / 33

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Solvents: In choosing a solvent, consideration must be given not only to its transparence, but also to its possible effects upon the absorbing system. Polar solvents such as water, alcohols, esters, and ketones tend to obliterate spectral fine structure arising from vibrational effects; spectra that approach those of the gas phase are more likely to be observed in nonpolar solvents such as hydrocarbons. In addition, the positions of absorption maxima are influenced by the nature of the solvent. Clearly, the same solvent must be used when comparing absorption spectra for identification purposes.

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