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Chapter 14 APPLICATION OF ULTRAVIOLET/VISIBLE MOLECULAR ABSORPTION SPECTROMETRY - page 29 / 33

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Procedural Details: The first steps in a photometric or spectrophotometric analysis involve the establishment of working conditions and the preparation of a calibration curve relating concentration to absorbance.

Selection of Wavelength: Spectrophotometric absorbance measurements are ordinarily made at a wavelength corresponding to an absorption peak because the change in absorbance per unit of concentration is greatest at this point; the maximum sensitivity is thus realized.

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