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Chapter 14 APPLICATION OF ULTRAVIOLET/VISIBLE MOLECULAR ABSORPTION SPECTROMETRY - page 30 / 33

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Variables That Influence absorbance: Common variables that influence the absorption spectrum of a substance include the nature of the solvent, the pH of the solution, the temperature, electrolyte concentrations, and the presence of interfering substances.

Cleaning and Handling of Cells: Accurate spectrophotometric analysis requires the use of good-quality, matched cells. These should be regularly calibrated against one another to detect differences that can arise from scratches, etching, and wear.

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