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Chapter 14 APPLICATION OF ULTRAVIOLET/VISIBLE MOLECULAR ABSORPTION SPECTROMETRY - page 31 / 33

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Determination of the Relationship Between Absorbance and Concentration: It is necessary to prepare a calibration curve from a series of standard solutions that bracket the concentration range expected for the samples. Ideally, calibration standards should approximate the composition of the samples to be analyzed not only with respect to the analyte concentration but also with regard to the concentrations of the other species in the sample matrix in order to minimize the effects of various components of the sample on the measured absorbance.

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