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W214

Effect of insulin-like growth factor-1 during culture on blastocyst mRNA abundance and survival in utero to day 14 of bovine embryos produced in vitro. J. Block*1, C. Wrenzycki2, D. Herrman2, T. M. Rodina1, H. Niemann2, A. D. Ealy1, A. E. Fischer-Brown3, and P. J. Hansen1, 1University of Florida, Gainesville, 2Institute for Animal Science, Neustadt, Germany, 3University of Illinois, Urbana.

W215

Effect of supplementation with Megalac-E on pregnancy rate in primiparous Nellore cows. C. N. Lopes1, J. L. M. Vasconcelos*1, T. P. B. Araujo2, and L. O. F. Oliveira3, 1FMVZ-UNESP, Botucatu, SP, Brazil, 2Arm&Hammer, Brazil, 3Propec Consultoria, Brazil.

W216

Progesterone postpartum determination and reproductive performance of crossbred cows. M. S. Arellano-Cornejo1, J. C. Martinez- Gonzalez*2, E. M. Romero-Trevino1, F. Briones-Encinia2, F. De la Garza-Requena2, and M. Dominguez-Munoz3, 1Instituto Tecnologico Superior de Altamira, Altamira, Tamaulipas, Mexico, 2Agronomia y Ciencias, Universidad Autonoma de Tamaulipas, Ciudad Victoria, Tamaulipas, Mexico, 3FMVZ, Universidad Autonoma de Tamaulipas, Ciudad Victoria, Tamaulipas, Mexico.

W217

Diagnosis of bovine freemartinism by fluorescence in situ hybridization using a bovine Y chromosome-specific DNA probe. S. H. Sohn, E. J. Cho, W. J. Son, and C. Y. Lee*, Jinju National University, Jinju, Korea.

W218

Influence of insulin on plasma and hepatic composition, ovarian activity and estrous behavior in early lactation dairy cows. J. A. Casas*, M. F. Sa Filho, C. Narciso, F. Rivera, and J. E. P. Santos, Veterinary Medicine Teaching and Research Center, University of California Davis, Tulare.

W219

Influence of parity on follicular dynamics and resumption of ovarian cycle in postpartum dairy cows. T. Tanaka*, M. Arai, S. Ohtani, S. Uemura, S. Kim, T. Kuroiwa, and H. Kamomae, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan.

W220

Pregnancy loss in lactating Holstein cows diagnosed with twin versus singleton fetuses. N. Silva del RÍo*1, J. D. Colloton2, and P. M Fricke1, 1University of Wisconsin, Madison, 2Bovine Services LLC, Edgar, WI.

W221

Effects of twin pregnancy and prepartum diet on early postpartum ovarian activity in Holstein dairy cows. N. Silva del Río*, R. R. Grummer, and P. M. Fricke, University of Wisconsin, Madison.

W222

Relationship between the occurrence of first ovulation in early postpartum and metabolic status in the cows that experiencing postpartum disease. M. Matsui*, E. Kaneko, M. Kataoka, C. Kawashima, N. Sudo, N. Matsunaga, M. Ishi, K. Kida, Y.-I. Miyake, and A. Miyamoto, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan.

W223

Effects of dietary fats differing in proportion of unsaturated fatty acids on characteristics of preovulatory follicles in dairy cows. M. Katz*1,2, A. Arieli2, and U. Moallem1, 1Agriculture Research Organization, Bet Dagan, Israel, 2Faculty of Agriculture, Hebrew University, Rehovot, Israel.

W224

Effects of endothelin-1 infused chronically adjacent the luteal-containing ovary or intrauterine in ewes on luteal function. C. W. Weems*, Y. S. Weems, D. Johnson, T. Uchima, E. Lennon, A. Raney, K. Goto, G. Bowers, J. Saldana, and J. Pang, University of Hawaii, Honolulu.

W225

Effect of extender on retention of viability and motility in hair sheep and goat semen stored at 4°C. J. L. Mook* and S. Wildeus, Virginia State University, Petersburg.

W226

Use of fecal progestin determinations to characterize the estrous cycle in captive female bontebok (Damaliscus pygargus pygargus). M. McGee*1, A. Kouba2, S. Bowers1, R. Meek2, B. L. Elliot2, C. Horton2, T. Hill2, E. Piorkowski2, and S. Willard1, 1Mississippi State University, Mississippi State, 2Memphis Zoo, Memphis, TN.

W227

Cloning and characterization of chicken prostaglandin E receptor subtypes 2 and 4 (EP2 and EP4). A. H. Y. Kwok*, C. Y. Wang, Y. Wang, and F. C. Leung, The University of Hong Kong, Hong Kong, HK-SAR, China.

W228

Pulmonary hemodynamic responses to intravenous prostaglandin E2 in broiler chickens. S. Stebel and R. F. Wideman, Jr.*, University of Arkansas, Fayetteville.

W229

Coordinate accumulation of the egg envelope glycoproteins during follicular development in Japanese quail (Coturnix japonica). T. Sasanami*1, M. Ohtsuki1,2, G. Hiyama3, N. Kansaku3, A. Tsukada4, K. Tahara4, T. Watanabe4, T. Yoshimura4, and M. Mori1, 1Shizuoka University, Shizuoka, Japan, 2Gifu University, Gifu, Japan, 3Azabu University, Sagamihara, Japan, 4Nagoya University, Nagoya, Japan.

W230

Nicarbazin reduces egg production and fertility in White Pekin ducks via reducing ZP3 in the perivitelline membrane. V. P. Reinoso*, R. Katani, and G. F. Barbato, The Pennsylvania State University, University Park.

W231

Isolation and culture of chicken oocytes. W. D. Berry*, S. S. Oates, L. M. Stevenson, and C. R. James, Auburn University Poultry Science, Auburn, AL.

W232

Gene expression of hen granulosa cell (GC) steroidogenic enzymes and gonadotropin receptors following a chronic heat stress (HS) episode. H. Taira*1 and M. M. Beck2, 1University of Nebraska, Lincoln, 2Clemson University, Clemson, SC.

W233

Some observations on molting male Japanese quail. B. K. Biswas and K. L. Arora*, Fort Valley State University, Fort Valley, GA.

W234

Rooster semen cryopreservation: Effect of line and male age on sperm function. D. C. Bongalhardo*1, J. Pelaez1, J. E. Fulton2, S. Saxena2, P. Settar2, N. P. O'Sullivan2, J. Arango2, and J. A. Long1, 1Beltsville Agricultural Research Center, Beltsville, MD, 2Hy-Line International, Dallas Center, IA.

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