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Validation of an on-farm tool (Z-Box) for determining a physical effectiveness factor using a bioassay based on chewing activity and ruminal fermentation in lactating dairy cows. H. M. Dann*1, K. W. Cotanch1, M. P. Carter1, C. S. Ballard1, T. Eguchi2, and R. J. Grant1, William H. Miner Agricultural Research Institute, Chazy, NY, 2Zen-Noh National Federation of Agricultural Co-operative Associations, Tokyo, Japan.



Use of a caliper to measure skinfold thickness in multiparous Holstein cows and its relationship to body condition score. H. M. Dann* and J. K. Drackley, University of Illinois, Urbana.


Development of a method for measuring forage fragility. K. W. Cotanch*1, R. J. Grant1, J. Darrah1, H. M. Wolford1, and T. Eguchi2, William H. Miner Agricultural Research Institute, Chazy, NY, 2Zen-Noh National Federation of Agricultural Co-operative Associations, Tokyo, Japan.



Near infrared spectroscopy can be used to predict pH and concentrations of volatile fatty acids in fermented feeds. D. P. Casper*, D. Spangler, J. Horst, S. Gravert, and K. Thompson, Agri-King, Inc., Fulton, IL.


Effect of lignin type, acid detergent lignin or Klason lignin, on rate and extent of NDF digestion. E. Raffrenato*, M. E. Van Amburgh, J. B. Robertson, and P. J. Van Soest, Cornell University, Ithaca, NY.


Estimating NDF rate of digestion: a comparison of different approaches for use in a first order model application. E. Raffrenato*, M. E. Van Amburgh, P. J. Van Soest, and J. B. Robertson, Cornell University, Ithaca, NY.


Urinary creatinine concentration during the periparturient period and the effect of correcting urinary creatinine concentration for DM content on the ability to predict total urinary output. G. Chibisa*1, G. B. Penner2, G. N. Gozho1, and T. Mutsvangwa1, 1University of Saskatchewan, Canada, 2University of Alberta, Canada.


New analytical method indicates that purine metabolites may interfere in estimates of microbial flow. S. M. Reynal* and G. A. Broderick, US Dairy Forage Research Center, Madison, WI.


Comparative characterization of reticular and duodenal digesta in dairy cows and possibilities to estimate microbial outflow from the rumen based on reticular sampling. A. N. Hristov*, University of Idaho, Moscow.


Kinetics of milk production as a function of energy and protein supplementation. R. P. Lana*1,2, D. C. Abreu1,2, P. F. C. Castro1, B. Zamperline1, and B. S. B. C. Souza1, 1Universidade Federal de Viçosa, MG, Brazil, 2CNPq, Brasília, DF, Brazil.


Effects of inoculation of ryegrass at ensiling on production of milk from dairy cows and whole body N partitioning. J. M. Moorby*, D. R. Davies, W. J. Fisher, and N. M. Ellis, Institute of Grassland and Environmental Research, Aberystwyth, UK.


Nitrogen utilization and nutrient digestibility in dairy cattle fed brown midrib corn silage and monensin. A. M. Gehman*, P. J. Kononoff,

and B. N. Janicek, University of Nebraska, Lincoln.


Effect of carbohydrates or amino acid infusions on plasma ghrelin in early and late lactating cows. I. Schei*1,2

and H. Volden1,

1Department of Animal and Aquacultural Sciences, Norwegian University of Life Sciences, Ås, Norway, 2TINE BA, Ås, Norway.


Depression in feed intake by a highly fermentable diet is related to plasma insulin concentration and insulin response to glucose infusion. B. J. Bradford* and M. S. Allen, Michigan State University, East Lansing.


Effect of weaning age on calving age, milk yield, and milk composition in the first lactation. J. A. Elizondo Salazar*, S. I. Kehoe, G. I. Zanton, C. D. Dechow, and A. J. Heinrichs, The Pennsylavania State University, University Park.


Effects of dietary AflaDetox on aflatoxin M1 residue in milk of dairy cows. M. Denli*1, J. C. Blandon1, S. Salado2, J. F. Perez2, and S. Calsamiglia1, 1Animal Nutrition, Management and Welfare Research Group, Universitat Autònoma de Barcelona, Barcelona, Spain, 2Adiveter S.L. Agro-Reus, Tarragona, Spain.


In vitro aflatoxin binding efficiency of several sequestering agents in water or rumen fluids. F. Masoero1, A. Gallo1, D. E. Diaz*2, G. Piva1, and M. Moschini1, 1Catholic University of Piacenza, Piacenza, PC, Italy, 2Utah State University, Logan.


Early lactation production, body condition, and incidence of disease in multiparous Holstein cows fed a low potassium diet supplemented with SoyChlor®16-7 prepartum. J. Siciliano-Jones1, P. W. Jardon2, M. Kucerak2, and M. B. de Ondarza*3, 1F.A.R.M.E. Institute, Homer, NY, 2West Central®, Ralston, IA, 3Paradox Nutrition, LLC, West Chazy, NY.


Intake of oral histidine does not alter milk or milk component production in dairy cattle. N. G. Purdie*, A. Krueger, V. R. Osborne, and J. P. Cant, University of Guelph, Guelph, Ontario, Canada.


Meta-functional genomics of the rumen biome. S. C. Fernando1, H. T. Purvis, II1, F. Z. Najar2, G. Wiley2, S. Macmil2, L. O. Sukharnikov2, T. G. Nagaraja3, C. R. Krehbiel1, B. A. Roe2, and U. DeSilva*1, 1Oklahoma State University, Stillwater, 2University of Oklahoma, Norman, 3Kansas State University, Manhattan.


A meta-analysis on the effects of feeding malate to ruminants. E. M. Ungerfeld* and R. A. Kohn, University of Maryland, College Park.


A multiple regression approach to explore the contribution of 2-hydroxy-4-methylthio butanoic acid or ruminally protected DL- methionine to production parameters for lactating dairy cows reported in the literature. G. R. Bowman*1, M. Vázquez-Añón1, and L. M.


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