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T305

Effect of a ruminal buffer and an amilolytic enzymes mixture added to a sorghum grain diet on finishing Criollo lambs. H. A. Lee– Rangel1, G. D. Mendoza–Martínez2, S. S. González*1, G. Ramírez–Valverde1, and J. H. Avellaneda–Cevallos3, 1Colegio de Postgraduados, Montecillo, Edo. México, México, 2UAM Xochimilco, México D.F., 3Universidad Técnica Estatal de Quevedo, Quevedo, Ecuador.

T306

Effects of exogenous amylase from Bacillus licheniformis on sheep performance and starch digestion. M. M. Crosby1, G. D. Mendoza*2, L. M. Melgoza2, J. R. Barcena1, and F. X. Plata2, 1Colegio de Postgraduados, Montecillo, Mexico, Mexico, 2Universidad Autonoma Metropolitana Xochimilco, Mexico, D.F., Mexico.

T307

Effect of feeding Fermenten on rumen fermentation in cows fed different concentrations of sucrose. G. B. Penner*1, L. L. Guan1, K. A. Beauchemin2, and M. Oba , University of Alberta, Edmonton, Alberta, Canada, 2Agriculture and Agri-Food Canada, Lethbridge, Alberta, Canada.

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T308

Influence of encapsulation of ascorbic acid to fermentation by rumen bacteria, in vitro. J. E. Garrett*1, G. Oenga1, A. Tayal1, and T. M. Webster2, 1Balchem Corporation, New Hampton, NY, 2West Virginia University, Morgantown.

T309

Quantification of Streptococcus bovis and Megasphaera elsdenii in ruminal fluid of dairy cows and beef heifers by real time PCR technique. M. Blanch*, S. Calsamiglia, and A. Castello, Universitat Autonoma de Barcelona, Spain.

T310

The effect of heat stress on rumen microbial composition analyzed by sequence-specific rRNA cleavage method. Y. Uyeno*1,3, Y. Sekiguchi1, K. Tajima2, A. Takenaka2, M. Kurihara2, and Y. Kamagata1, 1National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan, 2National Institute of Livestock and Grassland Science, Tsukuba, Japan, 3National Federation of Dairy Co- operative Associations, Tokyo, Japan.

T311

Application of carbohydrase inhibitors to moderate rumen fermentation: II. Continuous culture evaluation. S. M. Speight*1, D. L. Harmon1, and J. M. Tricarico2, 1University of Kentucky, Lexington, 2Alltech Biotechnology, Nicholasville, KY.

T312

Efficacy of Prevotella bryantii 25A and a mixture of Enterococcus faecium and Saccharomyces cerevisiae to control sub-clinical acidosis in dairy cows. J. Chiquette*1, M. J. Allison2, and M. A. Rasmussen3, 1Dairy and Swine Research and Development Centre, Lennoxville, Quebec, Canada, 2Iowa State University, Ames, 3SarTec Corporation, Anoka, MN.

T313

Differential effects of supplying reductant as hydrogen, formate or a combination of these on the methane-inhibiting activity of select nitrocompounds in vitro. N. A. Kruegar*, R. C. Anderson, T. R. Callaway, T. S. Edrington, R. B. Harvey, and D. J. Nisbet, USDA/ARS, Food & feed Safety Research Unit, College Station, TX.

T314

Effect of level of dietary malic acid supplementation on rumen methanogenesis and fermentation in beef cattle. P. Foley, J. Callan, D. Kenny*, T. Boland, and F. O'Mara, University College Dublin, Dublin Ireland.

T315

Usefulness of infrared imaging as a predictor of heat loss and methane production in dairy cows. Y.R. Montanholi*, N.E. Odongo, K.C. Swanson, F.S. Schenkel, B.W. McBride, and S.P. Miller, University of Guelph, Guelph, Ontario, Canada.

T316

Profiling energy substrate metabolism in isolated rumen epithelial and duodenal mucosal cells from beef cattle. S. W. El-Kadi*1, R. L. Baldwin2, K. R. McLeod3, N. E. Sunny1, S. L. Owens1, and B. J. Bequette1, 1University of Maryland, College Park, 2USDA-ARS, Beltsville, MD, 3University of Kentucky, Lexington.

T317

Rumen wall morphology and the change in bovine rumen absorptive capacity induced by varying digesta volume and pH. L. Q. Melo, F. Lopes, M. N. Pereira*, M. C. Guerreiro, S. F. Costa, and J. C. Resende Júnior, Universidade Federal de Lavras.

T318

Morphophysiologic evaluation of absorption and metabolism of volatile fatty acids by bovine forestomach. J. L. P. Daniel and J. C. Resende Júnior*, Universidade Federal de Lavras, Lavras, MG, Brazil.

T319

Evaluation of procedures for isolation of ruminant enterocytes. P. R. Regmi*, W. T. Dixon, and M. Oba, University of Alberta, Edmonton, AB, Canada.

T320

Ruminal, but not abomasal, infusion of starch differentially increases expression of concentrative nucleoside transporter (CNT) mRNA by small intestinal (SI) epithelia of forage-fed beef steers. S. F. Liao*, M. J. Alman, E. S. Vanzant, E. D. Miles, D. L. Harmon, K. R. McLeod, J. A. Boling, and J. C. Matthews, University of Kentucky, Lexington, KY, USA.

T321

Effect of hybrid (high starch content vs. high NDF digestibility) and maturity of corn silage on dairy cow performance. R.L.G. Zom*1, H.A. van Schooten1, and H. van Laar2, 1ASG Wageningen University & Research Centre, Lelystad, Netherlands, 2Nutreco Ruminant Research Centre, Boxmeer, Netherlands.

T322

Effects of a bacterial inoculant on fermentation, nutritive quality and degradability of corn, soybean and combined corn-soybean silages. L. O. Abdelhadi*1 and J. M. Tricarico2, 1Est. El Encuentro, Research & Extension in Ruminant Nutrition, Cnel. Brandsen, Argentina, 2Alltech Inc., Nicholasville, KY.

T323

Effects of a bacterial inoculant on fermentation, nutritive quality and degradability of combined corn-soybean silages in different geographical regions across Argentina. L. O. Abdelhadi*1 and J. M. Tricarico2, 1Est. El Encuentro, Research and Extension in Ruminant Nutrition, Cnel. Brandsen, Argentina, 2Alltech Inc., Nicholasville, KY.

T324

Effects of a bacterial inoculant on fermentation, nutritive quality and degradability of different sorghum silage hybrids. L. O. Abdelhadi*1

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