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CHOLESTEROL

Cholesterolum

C27H46O

Mr 386.7

DEFINITION

Cholesterol contains not less than 95.0 per cent of cholest-5-en-3β-ol and not less than 97.0 per cent and not more than 103.0 per cent of total sterols, calculated with reference to the dried substance.

ASSAY

Examine by gas chromatography (2.2.28), using pregnenolone isobutyrate CRS as the internal standard.

Internal standard solution. Dissolve 0.100 g of pregnenolone isobutyrate CRS in heptane R and dilute to 100.0 ml with the same solvent.

Test solution. Dissolve 25.0 mg of the substance to be examined in the internal standard solution and dilute to 25.0 ml with the same solution.

Reference solution. Dissolve 25.0 mg of cholesterol CRS in the internal standard solution and dilute to 25.0 ml with the same solution.

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