— resolution: minimum 1.5 between the peaks due to estragole and α-terpineol.
Using the retention times determined from the chromatogram obtained with the reference solution, locate the components of the reference solution in the chromatogram obtained with the test solution and locate cis-anethole and foeniculin using the chromatogram shown in Figure 2108.-1 (disregard any peak due to hexane).
Determine the percentage content of these components. The percentages are within the following ranges:
— linalol: 0.2 per cent to 2.5 per cent,
— estragole: 0.5 per cent to 6.0 per cent,
— α-terpineol: less than 0.3 per cent,
— cis-anethole: 0.1 per cent to 0.5 per cent,
— trans-anethole: 86 per cent to 93 per cent,
— anisaldehyde: 0.1 per cent to 0.5 per cent,
— foeniculin: 0.1 per cent to 3.0 per cent.