Wear heat-insulating gloves, safety glasses/face shield and closed toed shoes.
Ensure that the pressure of the chamber is ‘0' before opening the door.
Wear gloves and stand back from the door as a precaution, carefully crack door open no more than 1 inch
cm) to release residual steam and allow pressure within liquids and containers to normalize.
Allow sterilized material to stand for 10 minutes. This will allow steam to clear and trapped air to escape from hot liquids, reducing risk to operator. (Note: This is built into the operating cycle on some of the autoclaves).
Do not agitate containers of super-heated liquids or remove caps before unloading.
After removal from the autoclave, mark hot items with warning sign (e.g., “Caution: Hot. Do not touch.”) until the items cool to room temperature.
Push door or close the door for autoclaves with an automatic closure.
If any injury occurs seek first aid by contacting local designated first aider and/or Security Services at ext. 33333.
All incidents/accidents are to be reported to the designate responsible for the autoclave, the supervisor/manager and the Biosafety Officer and Insurance and Risk Management Services.
V. WORKING ALONE
If a staff member is working alone using the autoclaves during off-hours ensure appropriate contact with colleague and/or Security Services at ext. 33333
Spills may occur from a boil over or breakage of containers.
No operation of the autoclave is allowed until the spill is cleaned up.
Cracked glassware must be disposed of properly in the “Broken Glass” disposal pail.
Record the spill and cleanup procedure in the autoclave log book.
Seek further assistance from designate or additional staff if required.
MAINTENANCE, REPAIR and INSPECTION
The designate is responsible to report non-functioning autoclaves for repair or maintenance.
If an autoclave is not working properly, discontinue use immediately. Users are not to make repairs. A sign should be posted alerting others not to use the autoclave by the designate.
Autoclaves are considered unfired pressure vessels. They must be inspected at the time of installation by the Technical Safety and Standards Authority of Ontario (TSSA) and at least once in every 12 months thereafter depending on the type of autoclave. No person shall operate an autoclave unless it has been inspected by a Boiler Inspector holding a valid Certificate of Competency and a certificate of inspection has been issued. In
Revised: Nov., 2007