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6

A. tumefaciens

Right border of the Ti plasmid of A. tumefaciens LB 444 (White, 1992)

Ti plasmid of A. tumefaciens

Promoter for transcription of the nptII gene (White, 1992)

E. coli

Neomycin phosphotransferase type II enzyme that confers resistance to kanamycin and allows for selection in plant cells (Beck et al., 1982)

A. tumefaciens

A 3' nontranslated region of the nopaline synthase gene involved in transcription termination and polyadenylation (Depicker et al., 1982)

Cauliflower mosaic virus

Promoter that directs transcription of cryIA(B) gene (Odell et al., 1982)

Salmonella typhimurium

5-Enolpyruvyl-3-Phosphoshikimate Synthase Gene (Pittard, 1987)

see above

see above

A. tumefaciens

Left border of the Ti plasmid of A. tumefaciens (White, 1992)

E. coli

Resistance to ampicillin expressed only in E. coli (White, 1992)

Col E1 plasmid of E. coli

The origin of replication for the PUC plasmids that allows for plasmid replication in E. coli (Vieira and Messing, 1987)

E. coli

-galactosidase of E. coli (White, 1992)

lac

FUNCTION

Table 1—Summary of DNA components in pVSTI

Ori

RB

amp

GENETIC ELEMENT

SOURCE

LB

Indicate the functions of the gene(s), promoters, leader sequences, enhancers, introns, and any other sequences that are used for gene expression in the plant and a reference describing from where the sequences were obtained. Discussion should include whether the inserted sequences are responsible for disease or injury to plants or other organisms. The nucleotide sequence(s) of the plant-expressed gene(s) should be provided by citation and not submitted in the application. If there has been a significant modification to sequences and the modified se- quence has not been published, provide the complete sequence highlighting the modifications. If there have been minor modifications to the sequence of the plant-expressed gene, they should be provided. For example, if in a chemically synthesized Bt gene amino acid 23 was changed from methionine to alanine it should be stated without providing the complete sequence.

nos 5'

nptII

nos 3'

CaMV35s

aroA

nos 3'

SAMPLE PETITIONHerbicide-Tolerant Plants

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