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gene product, and demonstration that the whole plant is resistant to applied glyphosate (see subsequent sections).

B. The Selectable Marker Gene: Neomycin Phosphotransferase

In addition to the aroA gene, the nptII gene from transposon Tn5 of the bacterium E. coli has been introduced in cotton to be used as a selectable marker. This gene codes for the enzyme neomycin phosphotransferase which confers resistance to the common aminoglycoside antibiotic kanamycin (Fraley et al., 1986). The DNA sequence of the gene has been determined (Beck et al., 1982). The lack of risk to humans of the nptII gene can be supported by its use in the first human gene therapy trials (Anonymous, 1990). The nptII coding region is under the control of the nos promoter and nos terminator.

None of the introduced genes has any inherent plant pest characteristics or poses a risk to plant health when introduced into the modified plants.

SAMPLE PETITIONHerbicide-Tolerant Plants

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