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Biosafety Practices and Procedures - page 15 / 20

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15 / 20

Use sealed tubes, rotors, and safety cups/buckets that are sealed with O-rings

Inspect tubes, O-rings and rotors for wear, and buckets for cracks, chips, erosion, etc.

Do not use aluminum foil to cap centrifuge tubes

Clean and maintain gaskets and O-rings

Change O-rings if compromised

Load/unload centrifuge tubes, rotors and accessories in BSC

Small, low speed centrifuges may be placed in a BSC; however, high speed centrifuges pose additional hazards

Do not overfill tubes

Balance buckets, tubes and rotors properly before centrifugation

Wait 5 minutes (or 30 mins. for high speed centrifuge) after each run before opening

Do not decant or pour off supernatant.  Use a vacuum system with appropriate in-line reservoirs and filters

Safe Use of Centrifuge

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