The objective of this study was to investigate the effects of varying dietary levels of dry neem leaf meal (NLM) on the histology of testes and ovaries in Tilapia zillii fed for 60 days.
MATERIALS AND METHODS
Leaves were collected from neem trees in southwest Nigeria, where they occur naturally/planted as decorative plant, wind break or as a shade tree. They were shade-dried and milled into fine particle size (< 250 µm); and kept in a dry, clean, air-tight transparent plastic container. Feedstuffs were purchased from a local feedstuff market and were separately milled to small particle size (< 250 µm). A control diet (D 1, 350g crude protein/kg diet) was prepared as formulated in Table 1. Four test diets (D2, D3, D4, D5) were formulated by adding 5, 10, 15 or 20g of NLM to 1 kg of control diet, respectively. Nutrient imbalance caused by the addition of NLM was corrected by adding 2 g of cellulose (non-nutritive ingredient) to the basal diet (D1) and 1.5, 1.0, 0.5, and 0g of cellulose to test diets D2, D3, D4 and D5, respectively. The feedstuffs were thoroughly mixed in a Hobart A-200T mixing/pelleting machine. Hot water was added at intervals to gelatinize starch. All five diets were pelletized using a die of 8 mm diameter. The diets were air-dried at ambient temperature for 72 hours; broken, sieved into small pellet sizes, packed in air-tight containers, labelled and stored.
Table 1: Table 1: Ingredient composition of basal diet
Menhaden fish Meal
Cod liver Oil