Histology of ovaries in T. zillii fed varying dietary levels of neem leaf meal
Histological sections of the ovary in T. zillii fed with the control diet (containing no NLM) showed normal ovary histology. No pathological lesions were observed, atretic follicles were less visible (Figure 1), and normal olive green colour of ovaries was maintained. In fish fed 10 or 20g NLM/kg diet, there were changes in colour of ovaries, increased atretic follicles, ruptured follicles and necrosis (Figures 7 and 8).
Figure 6: Section of ovary in T. zillii fed diet 1 (control) showing normal histology and less visible atretic follicles. Mag. X 40.