gilent’s exclusive PicoTREC molecular recognition tool kit is designed for use with MC Mode. The option includes specialized hardware, electronics, consumables, and accessories. With PicoTREC, researchers can quickly distinguish between species that are engaged in molecular binding events and those that are not engaged in molecular binding events, thus eliminating the need to perform slow and tedious force-volume spectroscopy experiments to get the same results. Scientists can use PicoTREC with the 5500 FM to explore dynamic properties of biological systems (antibody-antigen, ligand-receptor, drug-receptor, DN-protein, DN-DN, and so forth) by imaging patterns of molecular binding and adhesion on surfaces.
Figure 8. PicoTREC imaging of the micropatterned avidin molecules. Topographic images (above, left), and corresponding recognition images (above, right).
Figure 9. Accuracy and reproducibility using PiocTREC anti-His3 on AFM and chromatin on glutaraldehyde mica. PicoTREC can repeat an experiment in a matter of minutes. In the top image blue indicates misses and red indicates false hits. In the bottom image green indicates hits. There was five minutes between scans and number of hits went down on the rescan (96% to 92%) and the false positive rate was 2.8%.
Images courtesy of Dr. Stuart Lindsay based on results generated in his lab by Dr. Hongda Wang at ASU.