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Only experienced personnel should use this route. Place rabbit in a snug restraining device. Sedation or topical anesthesia is recommended but not always necessary for experienced personnel. IF sedation or anesthesia is used, this should be administered and allowed to take effect before restraining the animal for blood collection.

  • 1.

    Intravenous injections are normally performed in the marginal ear vein. A normal iv injection is 500-1000 microliters. With the ear out straight, the vein is on the edge of the ear. By pressing lightly on the base of the ear, the return of the blood to the body can be restricted and the vein will stand out strongly. Some individuals may wish to pluck the hair over the injection site. Clean the injection area with 70% alcohol being injecting the mixture. Use of topical irritants to dilate the blood vessels is not allowed.

  • 2.

    Insert 22-25-gauge needle into the vein. The rabbit may twitch or move abruptly in response to being pricked by the needle, so make sure the animal is safely restrained. When the needle appears to be in the vein, gently pull back on the plunger. If blood

appears in the needle hub, slowly inject the contents of the syringe. seen, another location is selected.

If no blood is

3. After a short pause the needle is removed. A cotton or sterile gauze is placed over the

pressure is applied stop any bleeding. Wait 1-3 minutes to

to the

injection site. Holding the site tightly for a

few seconds will

verify

that any bleeding from the injection site will

not occur and no

4.

injection site as the needle is removed.

Once the syringe and needle are clear,

adverse reactions occur.

  • 5.

    Return rabbit to its cage.

  • 6.

    Follow post-injection monitoring procedures above.

For most laboratory animals the intravenous injection (iv) is a practical method of delivering a secondary or later boost. It is seldom used for primary injection. Antigen delivered directly in the blood stream will be processed rapidly by the reticuloendothelial system, primarily in the liver, lungs, and spleen. Normally, this procedure causes little pain and distress other than that accompanying the needle prick. However, iv injection does carry a low but potentially serious risk to the host animal, as it can induce pulmonary embolisms or lethal anaphylactic

shock in sensitized animals. Always

any

novel

substance

iv.

Any

toxic

consult with LAR material present

and/or in the

other experts before inoculum, such as

injecting bacterial

endotoxins, is particularly dangerous when delivered iv.

Physiological buffers and salt

solutions should be used if at all possible. Detergent concentration should never be allowed to exceed 0.1% for ionic detergents or 0.2% for non-ionic.

Test bleed on rabbits – marginal ear vein 1. Restrain the rabbit in an appropriate manner.

In general, a restraint device will be

used rather than a person merely holding the animal. Sedation or topical anesthesia is recommended but not always necessary for experienced personnel. If sedation or anesthesia is used, this should be administered and allowed to take effect before restraining the animal for blood collection.

  • 2.

    The marginal vein is found on the inner edge of the dorsal surface of the ear and should be visible. A patch over the vein about two-thirds of the distance from the head to the tip of the ear is gently plucked clean of hair or shaved. Prep the area with 70% alcohol or chlorhexidine solution.

  • 3.

    If the marginal vein is not visible or easily found, the ear may be gently heated under a low wattage lamp or by application of a warm compress. Use of topical irritants such as xylene to dilate the blood vessel is not permitted as it may cause skin damage.

Revised April 2007

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