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4.

Blood collection is done only with a sterile needle and syringe or butterfly catheter and syringe. The ear is held out horizontally and stabilized. Penetrate the vein with the needle bevel up. Only a small portion of the needle is inserted parallel above the vessel with the tip directed into the lumen along with the longitudinal axis. Sampling of

blood from

the vein

Additional

attempts

should be performed as close can be made distally toward

to the base the ear tip

of the ear as possible.

for

the

vein.

Proper

insertion of the needle guidance and training

into can

the vein is the most important part of the procedure. While be provided, individuals should understand that practice is

5.

necessary in order to achieve proficiency. Withdraw the amount of blood desired but

bear

in

mind

that

only

small

volumes

(less

than 3 ml) will be obtainable from the marginal ear vein due to its size. If amounts are necessary, then blood should be collected from the central ear

Also keep in mind that rabbits are capable

vessels,

particularly

when

distressed.

Use

of of

constriction of their peripheral a topical anesthetic such as

larger artery. blood EMLA

6.

7. 8.

cream or lidocaine jelly helps minimize this reaction. Following completion of blood withdrawal, remove penetration site with a cotton ball. Apply gentle minutes to ensure adequate hemostasis. Verify that no further bleeding is occurring and return

Follow post-injection monitoring procedure above.

the needle and cover pressure to the site for

rabbit to its cage.

the

vein

up

to 2

Note: Blood collection from rabbit ears by transecting or cutting the vein s not permitted. Never use a scalpel or needle bevel to cut the vessels. Always insert a needle into the

vessel.

Blood collection rabbits – central ear artery 1. Restrain the rabbit in an appropriate manner.

In general, a restraint device will be

used rather than a person merely holding the animal. Sedation or topical anesthesia is highly recommended but not always necessary for experienced personnel. If sedation or anesthesia is used, this should be administered and allowed to take effect before restraining the animal for blood collection. Recommendation for anesthesia is (1) 0.2- 1.0 mg/kg acepromazine SC + topical 2% lidocaine HCl jelly or EMLA cream or (2) 1.0 mg/kg acepromazine + 1.0 mg/kg butorphanol SC. 2. The central artery is easily located on the dorsal surface of the ear and should be visible. Plucking or shaving is not necessary but may be done if desired. Prep the

area with 70% alcohol or chlorhexidine solution. 3. Sedation or anesthesia should dilate the artery for ease of collection.

If not using

4.

sedation or anesthesia and the artery appears to be small or constricted, the ear may be gently heated under a low wattage lamp or by application of a warm compress. Use of topical irritants such as xylene to dilate the blood vessel is not permitted as it may cause skin damage. Blood collection is done only with a sterile needle and syringe, butterfly catheter and syringe or vacutainer needle and tubes. The ear is held out horizontally and stabilized. Penetrate the artery with the needle bevel up. Only a small portion of the needle is

inserted

parallel

above

the

vessel

with

longitudinal

axis.

Sampling

of

blood

performed as close to the tip of the ear

the tip directed into the lumen along with the from the artery, unlike the vein, should be as possible. Additional attempts can be made

closer to the base of the most important provided, individuals

the ear if needed. Proper insertion of the needle

part

of

the

procedure.

While

should understand that practice

guidance and is necessary in

into the training order to

artery is can be achieve

Revised April 2007

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