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Note: The Norwegian offers video Reference Centre for Laboratory Animal Science and Alternatives has slide/video demonstration of this technique on line.

REFERENCES

Niemi, S.M., Fox, J.G., Brown, L.R., and Langer, R.

Evaluation of ethylene-vinyl acetate

copolymer as a non-inflammatory alternative to Freund's complete adjuvant in rabbits. Lab. Anim. Sci.; 35(6):609-612, 1985. Diano, M., LeBivic, A. and Hirn, M. A method for production of highly specific polyclonal antibodies. Analytical Biochemistry; 166:224-229, 1987. Halliday, L.C., Artwohl, J.E., Hanly, W.C., Bunte, R.M. and Bennett, B.T. Physiologic and Behavioral Assessment of Rabbits Immunized With Freund’s Complete Adjuvant. Contemporary Topics, 39 (5):8-13,2000. Halliday, L.C., Artwohl, J.E., Ramakrishnan, V., Bunte, R.M. and Bennett, B.T. Effects of Freund’s Complete Adjuvant on the Physiology, Histology, and Activity of New Zealand White

Rabbits. Contemporary Topics, 43 (1):8-13,2004.

Leenaars, M and Hendriksen, D.F.M. Monoclonal Antibodies: Evaluation and

Critical Steps in the Production of Polyclonal Recommendations. ILAR Journal, 46 (3).

and

Schunk, M.K. and Macallum, G.E.

Applications and Optimization of Immunization

Procedures. Stills, H.F.

ILAR Journal, 46 (3). Adjuvants and Antibody

Production: Dispelling the Myths Associated with

Freund's Complete and Other Adjuvants. ILAR Journal, 46 (3).

Revised April 2007

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