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Nonradioactive Hybridization Procedures

Use of biotin-labeled nucleotides in DNA probes instead of 32P, then add avidin (streptavidin) which binds to biotin, and then add biotin attached to an enzyme like alkaline phosphatase for detection (see Fig. 9.11)

Note that fluorescent dyes can also be attached to DNA primers for detecting amplified DNA products (see Fig. 9.12)

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