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Fig. 9.1 Enzyme-Linked Immunosorbent Assay (ELISA): immunological detection

Target molecule

antigenic site

Support

A. Bind sample to the support (commonly plastic or a membrane)

B. Add primary antibody; wash

C. Add secondary antibody-enzyme conjugate; wash

D. Add substrate

bound primary

antibody

E

E

enzyme linked

secondary antibody

colorless substrate

colored product

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