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Fig. 9.3 Explanation of how HAT medium works

Myeloma cells are HGPRT- and will die on HAT media having hypoxanthine,

aminopterin (an antifolate), and thymidine.

Spleen cells are HGPRT+ , so spleen-myeloma (hybridoma) cells can grow on HAT.

(Note: spleen cells by themselves cannot grow in culture.)

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