while less lignified cells take up an orange colour (Fig. D and E). Cellulose was localized using Calcofluor white (Fig. I). Only parenchymatous cells showed high intensity of fluorescence, indicating their cellulose-rich nature.
Fig. G. Section stained with acridine orange and observed under fluorescent microscope with blue excitation, AO shows high affinity for lignin that appears bright yellow, X 200; Fig. H. Section stained with Coriphosphine O under blue excitation, lignin rich regions appear bluish yellow to bright yellow, phloem appears blue in colour, X 200; Fig. I. Section stained with Calcofluor White, cellulose rich region fluoresce and appear blue in colour, X 200; Fig. J. An enlarged view of fibrovascular bundle stained with AO, X 400
Chemical composition of culm strands The processed culm strands obtained from silk mat weavers of Pathamadai were analyzed for chemical properties. The culm strand consist the following chemical constituents; ♦ Holocellulose – 82.92% ♦ Alpha cellulose – 41.79% ♦ Hemicellulose – 41.13% ♦ Lignin – 13.28% ♦ Waxes – 1.73% ♦ Moisture – 9.2%
Fibre dimensions and derived values
Four different types of fibres were identified based on the relative thickness of the cell walls seen by staining the macerated fibres of rind and core regions. The observations showed significant variations between the rind and core fibres. The rind region showed the presence of very thick walled (vtkf) and thick walled fibres (tkf) (Figs.
Benazir et al. (2010). “Sedge fibers and strands,” BioResources 5(2), 951-967.