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Shekhar M. Bhavsar et al _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ J. Chem. Pharm. Res., 2010, 2(2): 563-572 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

responsible for the production of Prostaglandin in the body. Lornoxicam is chemically 6-chloro-4-

hydroxy-2-methyl-N-2-pyridyl-2H-thieno-[2, (Fig.1) [1].

3-e]-1,

2-thiazine-3-carboxamide-1,

1-dioxide

Thiocolchicoside (Molecular Formula: C27H33NO10S, Molecular Weight: 563.618 gmol-1) is a Centrally Acting Muscle Relaxant. It is used in the treatment of Painful Muscle Spasm [3]. Thiococlchicoside is chemically N-[3-(β-D-glucopyranosyloxy)-1, 2-dimethoxy-10-(methylthio)- 9-oxo-5, 6, 7, 9-tetrahydrobenzo[a]heptalen-7-yl] acetamide (Fig. 2) [4].

Literature review revealed that, both Lornoxicam and Thiocolchicoside are not official in any pharmacopoeia. On detailed literature survey, it was found that only few selected RP-HPLC, LC and LC/MS/MS methods were reported for estimation of Lornoxicam [5, 6, 7, 8, 9, 10] and Thiocolchicoside [11, 12, 13] in their individual dosage form and plasma samples. These methods are not so specific for simultaneous estimation of Lornoxicam and Thiocolchicoside in their combined dosage form. So, this is the first report of RP-HPLC method for the estimation of Lornoxicam and Thiocolchicoside in combined dosage form. The method was validated as per ICH guidelines [14].

Fig.1: Structure of Lornoxicam

Fig. 2: Structure of Thiocolchicoside

Materials and Methods

Experimental Lornoxicam, Active Pharmaceutical Ingredient (API) and working standard was supplied by Glenmark Generics Limited. Thiocolchicoside, Active Pharmaceutical Ingredient (API) and working standard was supplied by Alchem International Limited. Combination drug product of Lornoxicam and Thiocolchicoside was provide by Zydus Cadila Healthcare Limited (Ahmedabad, India)

Chemicals and reagents used Ammonium Dihydrogen Phosphate: Merck Specialities PVT Ltd.

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