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Shekhar M. Bhavsar et al

J. Chem. Pharm. Res., 2010, 2(2): 563-572

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Tri Ethyl Amine: Spectrochem PVT Ltd., Mumbai. Methanol: For HPLC, Spectrochem PVT. Ltd., India Milli-Q Water: In-house production of company

Apparatus and equipments used Hot air oven: Proto – Tech oven. Analytical Balance: AX 205, METTLER TOLEDO. pH Meter: Thermo Orion, model 420. Sonicator: Oscar Ultra Sonics, OU- 72 (SPL).

Chromatographic condition

The HPLC system (Shimadzu Corporation, Japan), model Shimadzu VP, consisted of a system controller (CLASS-VP), on-line degasser (LC 2010C, Shimadzu), low pressure gradient valve (LC 2010C, Shimadzu), solvent delivery module (LC 2010C, Shimadzu), auto injector (LC 2010C, Shimadzu), column oven (LC 2010C, Shimadzu), and CLASS – VP software version==SPI, binary pump, auto injector (SIL-10AD VP, Shimadzu), column oven (CTO-10AS VP, Shimadzu) and PDA detector (PDA-SPD-M10A VP, Shimadzu Diode Array Detector) and Chem station (software).

The chromatographic parameters

Column

: Inertsil ODS 3V C-18 (250 x 4.6 mm), 5 µ

Detector

: 290 nm

Injection Volu Flow Rate Temperature Run Time Mobile Phase Diluent

me

: 10 µl : 1.5 ml/min : 30ºC : 15 minute : Buffer: Methanol (45:55) : Mobile phase

Buffer preparation Dissolve 5.7606 gm of Ammonium Dihydrogen Phosphate in 2000mL of milli-Q water and adjust pH of this buffer solution to 7.3 with Tri Ethyl Amine.

Preparation of standard solution The standard stock solution was prepared by transferring 40 mg of Lornoxicam and 40 mg of Thiocolchicoside in a 200 mL volumetric flask. Add about 50mL of mobile phase and sonicate to dissolve. Now make volume up to mark with diluent. Dilute 10mL of this solution to 25mL with Mobile phase and mix. Final standard concentration of Lornoxicam and Thiocolchicoside is 80ppm.

Preparation of test solution Accurately 20 intact tablets were weighed to determine average weight of tablets. Then tablets were finely crushed and tablet powder equivalent to about 40mg of Lornoxicam was transferred into 200 mL volumetric flask. Then 100 mL diluent was added to flask and sonicated for 30 minutes with intermittent shaking. Make the volume up to mark with Mobile phase and mix. Solution was filtered through 0.45 µ HVLP millipore filter; collect the filtrate by discarding first

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