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Compound

Retention Time

n

R

T

k’

Thiocolchicoside

2.95 ± 0.0048

2943.34

0.00

1.11

28.65

Lornoxicam

9.39 ± 0.0074

6368.75

18.55

1.20

93.08

Shekhar M. Bhavsar et al

J. Chem. Pharm. Res., 2010, 2(2): 563-572

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ System suitability and system precision _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

System suitability and system precision was daily performed during entire validation of this method. The results of system suitability and system precision were presented in table 1.

Table 1: System suitability and system precision

Table 2: Results of Linearity

T o a c h i e v e l i n e a r i t y a n d r a n g e , s t o c k s o l u t i o n c o n t a i n n= Theoretical plates; R= Resolution; T= Asymmetry, k’= Capacity Factor. Linearity i n g 0 . 2 0 0 mg m L-1 L o r n o x i c a m a n

d 0 . 2 0 0

m g m L - 1 T h i o c o l c h i c o s i d e w e r e d i l u t e d t o y i e l d s o l u t i o n i n t h e c o n c e n t r a t i o n r a n g e o f 0 . 2 4 1 2 0 µ g m L - 1 a n d 0 . 2 3 5 1 2 0 µ g m L - 1 f o r L o r n o x i c a m a n d T h i o c o l c h i c o s i d e , r e s p e c t i v e l y . T h e solutions were prepared in triplicate and analyzed by using 10 µl into HPLC. The results related to linearity are presented in Table 2.

Linearity Range

0.24 – 120 g mL-1

0.235 – 120 g mL-1

Linearity equation

y = 29738.77 x + 2700.34

y = 18227.53 x -3713.71

Correlation coefficient

0.9999

0.9999

LOD

0.8 g mL-1

0.8 g mL-1

LOQ

0.24 g mL-1

0.23 g mL-1

Parameters

Lornoxicam

Thiocolchicoside

LOD= Limit of detection, LOQ= Limit of quantification, RT=Retention time.

Fig. 3: Linearity and calibration curve of Lornoxicam

567

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