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placed in a mating disrupted area should catch no to very low levels of male insects. The 2005 data (Figure 2) indicates that all treatments were equally effective in maintaining trap shutdown.

Figure 2. 2005 Seasonal trap capture of Oriental beetle adult males.

Virgin females were also used to test treatment effectiveness. Mating estimates replace data that could be gathered by the destructive sampling of commercial bushes for presence of larvae. From a lab culture, fresh virgin females were separated from males. Two to three virgin females were placed in each of  4-5 cage-pots, located near the center of each plot, and were retrieved after 1-3 days. Cages were placed on July 6, 12, 19, and 22. After retrieval, data was taken for: the number of females and males recovered per cage. Individual females were placed in 1oz plastic cups with moist soil and checked for eggs once per week for 3 weeks. Resulting eggs (usually after 7-10 days) were incubated at 25oC and checked biweekly for development and hatching for up to 3 weeks. When adults died, they were held 10 oC. All females in cages without males were dissected for the presence of eggs, and eggs incubated as above. Females with males that had not laid eggs were also dissected, and any eggs removed as above. For any egg cluster in which at least 1 egg hatched, the cluster was classified “viable” resulting from a fertilized female. For all practical purposes, males were recovered only from control cages. Practically all fertile eggs resulted from females found with males in cages (mated females) in the controls. One male was found in a treatment cage (medium rate) on the 2nd deployment. Eggs dissected from 1 female in a low treatment cage (no males) did hatch, inferring that there was a male that probably escaped. Data indicates that all treatments were equally effective in shutting down pheromone communication.

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