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Examine the data in Figures 1 and 2. Come to consensus within your group about the following questions:

  • 1.

    What is the degree of genetic relatedness among each of the isolates in this experiment?

  • 2.

    o which isolate is the isolate carried by Kayla most closely related?

  • 3.

    Noting the differences in DNA banding patterns below, explain how these MRSA isolates can be susceptible to the

same antibiotics? o different antibiotics?

Figure 1. Description of Samples in Each Lane

Lanes  and —Hospital-acquired MRSA isolates; both patients had multiple previous hospitalizations. Both were susceptible only to vancomycin.

Lanes–—MRSAisolatesobtainedfrompediatricpatients without any known risk factors for MRSA. Antibiotic susceptibility includes trimethoprimsulfamethoxazole, tetracycline, and vancomycin.

Lane —Isolate from Dr. Collin’s patient.

“Dr. Collins and the Case of the Mysterious Infection” by Lemons & Huber

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