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Description of Oil

Published Protein

Separation Method

Refined, deodorized Crude

Yes Yes

Chromatography Aqueous Extraction

“Processed” Uncharacterized, commercial

Yes

Aqueous Extraction

Fully refined, commercial

No

Unknown

Appendix 3: Evaluation of Published Measurements of Protein Concentrations in Oils.

Oil Type

Soy

Reference

a

Tattrie and Yaguchi, 1973 Klurfeld and Kritchevsky, 1987

Awazuhara et al., 1998

Reeves, 1999

Protein

0.72

0.014 0.017 0.018 0.023 0.027 0.040 0.033 0.042 0.049 0.057 0.082 0.114 0.222

Concentration (ug/g)

0.96 1.93

Protein Quantitation

Method Amino Acid Analysis Commercial Bradford Assay

Lowery Assay

Amino Acid Analysis

0.0332 0.0353

Refined

0.0898 0.1010 0.1380 0.32

Unrefined

Deodorized

1.80

Cold pressed

Paschke et al., 2001

Errahali et al., 2002

Yes

Acetone Precipitation

Yes

Aqueous Extraction

Bradford Assay

Unknown

Revised Threshold Report

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