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Appendix 3: Evaluation of Published Measurements of Protein Concentrations in Oils.

Oil Type

Reference

a

Protein

Concentr

ation

Description of Oil

Published Protein

Separation

Protein Quantitation

Nordlee et al., 2002

(ug/g) 0.16 – 20.8

Degummed

No

Method Aqueous Extraction

Method Amino Acid Analysis

0.043 – 6.8

Refined

0.033 – 3.1

Bleached

Peanut

Cold pressed

Yes

Aqueous Extraction

Commercial Coomassie Dye Assay

Refined, bleached, deodorized

Yes

Aqueous Extraction

Commercial Bradford Assay

Unrefined

Refined, commercial

Yes

Aqueous Extraction

Commercial Bicinchoninic Acid (BCA) Assay

Crude

No

Aqueous Extraction

Lowery and Commercial BCA Assay

60

Klurfeld and Kritchevsky, 1987

Hoffman and Collins- Williams, 1994

Teuber et al., 1997

Olszewski et al., 1998

Skinner and Haynes, 1998

0.021 – 0.443 0.120 0.154 0.204 0.206 0.580

0.2 0.6 3.3 3.3 3.0 ± 0.3

    • 5.7

      ± 1.2

    • 10.5

      ± 0.4

    • 10.7

      ± 0.8

0.10 0.13 0.15 0.16 0.20 187

Deodorized Processed

Yes

Aqueous Extraction

Bradford Assay

Alkali refined, neutralized

Revised Threshold Report

Page 102 of 108

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