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Appendix 3: Evaluation of Published Measurements of Protein Concentrations in Oils.

Oil Type

Reference

a

Teuber et al., 1997 (Walnut)

Protein Concentration

Description of Oil

(ug/g) 7.0 ± 2.5 7.0 ± 0.8 9.2 ± 3.1

Refined, bleached, deodorized

Published Protein Separation

Protein Quantitation

Yes

Method Aqueous Extraction

Method Commercial Bradford Assay

16.5 ± 2.4

Unrefined

20.4 ± 1.8

Blend

Note: Protein levels too low to detect or measure were reported by Tattrie and Yaguchi (1973), Hoffman and Collins-Williams (1994), Yeung and Collins (1996), Peeters et al. (2004) for peanut oils and by Tattrie and Yaguchi (1973), Porras et al. (1985) for soy oils. These values were not included due to the lack of methodological information.

a None of the publications provide sufficient information to evaluate the overall extraction efficiency, accuracy, reproducibility, or precision of the method used. In addition, in most cases, it was not clear whether replicate samples were tested or whether replicate measurements were carried out for individual samples.

b Crevel et al. (2000) is a review paper that includes previously unpublished data. These data are given here, but are considered unpublished because the research that generated these values has not specifically been peer-reviewed.

Revised Threshold Report

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