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Appendix 1: Evaluation of Commercially Available Analytical Methods for Proteins from Major Food Allergen

Validationb

Sensitivity (LODc) (ppm) Not reported

Quantitation (LOQc) (ppm)

No

Not reported

JRC No No

0.5 0.5 5

1 1 No

No

5

No

AOAC MLPT

Not reported

2.5

No

2.5

Not reported

AOAC MLPT

1.5

2.5

No

0.5

2.5

AOAC MLPT

0.1

1

No

Not reported

No

No

Not reported

0.16

No

0.5

1

No

1

1

Elisa Systems

Peanut

Elisa Systems

Total Peanut

Neogen

Alert for

Peanut

Neogen

Reveal for

Peanut

Neogen

Veratox for

Peanut

Yes

Peanut

Ara h2

Yes

Yes

Peanut

Total

Not

Not reported

Not reported

Yes

Allergen

Manufacturera

Method

Peanut

Abkem Iberia

Peanut DiagnoKit

Not reported

Not reported

Yes

Peanut

Not reported

Yes

Not reported

Total

Yes

Peanut

Total

Yes

Not reported

Not reported

Yes

Peanut

Conarachin (Ara h 1)

Yes

Not reported

Not reported

Yes

Not reported

Casein

Yes

Not reported

Beta- Lactoglobulin Beta- Lactoglobulin

Yes

Not reported

Yes

Milk

Raw and Processed Foods? Yes (for

specified foods)

reported Not reported Yes (for specified foods) Yes

Yes (for specified foods) Yes

Yes (for specified foods) Not reported Yes (for specified foods) Yes

Yes

Species Specificity

Protein(s) Detected

Practicality

Not reported

Not reported

Yes

Tecra

Peanut Visual

Immunoassay

Tepnel

BioKits Peanut

R-BioPharm

R-BioPharm

RIDASCREEN Peanut RIDASCREEN FAST Peanut

Tepnel

Abkem Iberia

BioKits Rapid Peanut Casein DiagnoKit

Elisa Systems

Elisa System

Beta- lactoglobulin Enhanced Beta- lactoglobulin

Revised Threshold Report Page 85 of 108

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